• Question: how are cultures done

    Asked by 762bm1928 to Peter, Grace, Doris, David on 28 May 2019.
    • Photo: Doris Nyamwaya

      Doris Nyamwaya answered on 28 May 2019:

      Blood culture is used to detect if there are any microorganisms in your system that would cause disease. Blood is drawn from the patient, then mixed with a medium/ broth that contains all requirements essential for the growth of various microorganisms e.g bacteria, fungi. If any of these successfully grows, they are either physically identified using known characteristics , or further scrutinized using other methods like microscopy

    • Photo: Grace Kago

      Grace Kago answered on 28 May 2019:

      First, let’s define what a biological culture is: a culture is a technique used by scientists to grow microorganisms in a controlled setting. There are a few key steps to remember when making a culture:
      1. In order to prepare a culture, you need to know which conditions the microorganism likes, and in which condition it will grow the best. If it is your first time to grow the culture, you can read how other people have grown that organism, or you can try and mimic its natural environment.
      2. You need to make sure that the solution that you are working with is super clean (another word for super clean= sterile), because you have to make sure that only your organism of choice grows in your solution.
      3. Once you grow/cultivate your culture, you need to determine a way to examine or measure your organism. For example, with bacteria, you can measure their quantity by quantifying the absorbance at 600nm of your culture. Another way is by adding certain dyes that “paint” your bacteria and then you can measure the dye to know how many bacteria you have. With larger organisms, you can look at them under a microscope, etc.

    • Photo: Peter Macharia

      Peter Macharia answered on 29 May 2019:

      A blood culture is usually done to see if there are any foreign unwanted things in your blood, say for example a bacteria, or other microorganisms. This means if the culture is positive, you have bacteria in your blood.

      So how is it done?

      Blood is drawn from a person and then mixed with materials that create a conducive environment for the microorganisms to grow. After some time, the examine the microorganism to determine the next course of action.